전기적 자극이 백서 대퇴골골단의 연골형성에 미치는 영향에 관한 연구
AN EXPERIMENTAL STUDY ON THE CHONDROGENESIS INDUCED BY CONSTANT DIRECT CURRENT WITH LOW AMPERAGE IN RAT FEMUR
소속 상세정보
고천석/Chun -Suk Ko
KMID : 0358919900170010037
Abstract
결론
저자는 전기적 자극이 백서 대퇴골두 골단연골의 연골형성에 미치는 영향을 관찰하기 위하
여 생후 3개월된 체중 200gm 내외의 웅성백서 20마리를 실험군 15마리, 대조군 5마리로 분
류 하였다. 실험군은 우측 대퇴골 근위단에 stainless steel wire로 된 음극선을 장착시키고,
척추와 양측의 장골능을 연결하는 선이 만나는 지점에 양전극을 피하매식하여 전원에 연결
한 후 7-l8μA의 직류전기를 6, 12시간, 1일, 3일, 7일간 적용하였으며 각 군당 3마리씩으로
하였다. 대조군은 실험군과 동일한 방법으로 음전극 및 양전극을 장착시켰으나 전월에는 연
결시키지 않았으며 각 군당 1마리씩으로 하였다. 각 적용시간 경과 후 모든 동물을 회생시
켜 광학현미경과 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다.
1 전기적 자극에 의해 연골내 화골이 촉진되었다.
2. 골단성장대 연골세포의 활성화는 6시간군에서 높아졌으며, 12시간 군에서 현저하였다.
3. 이차골화중심이 3일군의 골단성장대 외의 예비연골대에서도 발생되어 골화가 화대되었
으며, 7일군의 골단연골은 비박되어 대조군과 차이가 없었다.
4. 파골세포의 활성도는 1일 군에서 높았으며, 골아세포의 활성도는 3일 군에서 높아 신생
골형성이 현저하였다.
5. 연골아세포의 활성화는 0.127 Jou1e에서 나타났으며, 0.332 Joule에서 현저한 연골 형성
이 나타났다.
#초록#
The purpose of this study was to investigate the changes in the chondrogenesis
processes, induced by a direct eclectic current ranging 7-18μA, of rat femur. 20
rats(Sprague-Dawley strain) weighing about 200gm were selected and divided into 2
groups, one control group consisting of 5 rats and one experimental group consisting of
15 rats. And the control and experimental group were subdivided into 5 groups
according to the periods (6-hour, 12-hour, 1-day, 3-day, 7-day) that the electric
stimulation applied. All surgery was performed under ether and thiopental sodium
anesthesia with aseptic precautions, each animal receiving 0.25cc/100gm of soluble
gaiamycin sulfate intraperitoneally at the end of the surgical procedure.
A 0.010' stainless steel wire covered with polyethylene tube was tied at the right
femoral neck portion of the rat, and this wire serving as a cathode was connected to
the Constant Current Sink. Anode was implanted subcutaneously over the dorsum at the
cross section area where the vertebral column and a line connecting the both iliac
crests, and then connected to the Sink. Control groups were received the same
electrodes but that those electrodes were not connected to the power supply pack.
Animals were sacrified at 6-hour, 12-hour, 1-day, 3-day, and 7-day after the electrical
stimulation. Head of femoral condyle was prepared for histologic findings including LM
and EM.
Based on the present study the following conclusions can be made .
1. It can be concluded that the activity of the chondroblasts was stimulated by means
of direct electric current.
2. The increased activity of chondroblasts in the epiphyseal plate was noted at the
6-hour experimental group, and a marked increased activity was evident at 12-hour
experimental group.
3. There appeared a secondary ossification center in the prechondroblastic layer at
3-day experimental group, and the epiphyseal plate of the 7-day experimental group
showed an insignificant change relatively to the 7-day control rat.
4. The increased activity of the osteoclasts was marked at 24-hour experimental group,
and the new bone formation was remarkable owing to the increased activity of the
osteoblasts at 3-day experimental group.
5. The total energy dissipated approximately 0.13 joule elicited a marked changes of
chondroblasts in the epiphyseal plate, and a remarkable bone formation was produced at
about 0.33 joule.
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